Journal: American Journal of Physiology-Lung Cellular and Molecular Physiology

Article Title: Mechanical distension modulates pulmonary alveolar epithelial phenotypic expression in vitro

doi: 10.1152/ajplung.1998.274.2.l196

Figure Lengend Snippet: Fig. 3. Effect of mechanical distension of type II cells on content of mRNA for rTI40. Southern blots were performed from RNA obtained from unstretched control (C) and stretched (S) cells that was sub- jected to reverse transcription-polymerase chain reaction (RT-PCR) for rTI40 as described in METHODS. Autoradiograms (shown above columns) were obtained before quantification of radioactivity by volume integration of pixel values as measured by phosphorimage analysis; first 2 lanes of each represent duplicate control samples and next 2 lanes represent duplicate stretch samples. Glyceraldehyde-3- phosphate dehydrogenase (GAPDH) was used as a control to ensure quantitative recovery of cDNA after RT. Results are expressed as percent change from unstretched controls, means 6 SD of 3 experi- ments, each with a different preparation of cells. *P , 0.05 compared with respective control cells.

Article Snippet: Filters were then subjected to autoradiography as described above; quantitation of radioactivity was performed by volume integration, using phosphorimage analysis (Imagequant, Molecular Dynamics).

Techniques: Control, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Radioactivity